Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122522_84ju GATGTCTGGAGAGCAAATCCCCAATAGGCTTG(+AGGACA)GATCCTCAGAACCGGTCACCGACCCAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613122521_w713 TGGCTCTTCCTGGGTCGGTGACCGGTTCTGAG(+AGGACA)GATCCAAGCCTATTGGGGATTTGCTCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613122519_9i1y GGGTAGGAATTGAAGACATGGTCACACCTCAG(+AGGACA)GTACCAGTTTCTGCTCTGCCAGGCGCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613122518_4i3w AGTGTAGAAAGCGCCTGGCAGAGCAGAAACTG(+AGGACA)GTACCTGAGGTGTGACCATGTCTTCAATTCC NGG 10 8 17 8 24 0 100 5 10
    240613122516_rqyc GTACTCCGGGCTGCCCCAGAAACCTGCCTTTG(+AGGACA)GATCCATCTTGTCTGTTTAGAGAGAGCGGAG NGG 10 8 17 8 24 0 100 5 10
    240613122515_aq9p GGGCACTCCGCTCTCTCTAAACAGACAAGATG(+AGGACA)GATCCAAAGGCAGGTTTCTGGGGCAGCCCGG NGG 10 8 17 8 24 0 100 5 10
    240613122513_dyqd GGATGTCCTTCCAGGTCAATAATCTTAGCAGG(+AGGACA)GTACCTTCCTCAGAATGGCCAGAGCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613122512_7ig5 CTCCTTCTCCAGCTCTGGCCATTCTGAGGAAG(+AGGACA)GTACCCTGCTAAGATTATTGACCTGGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613122510_d0ze CACCAGAAAGAGATCATGATGTGCCAGCCAGG(+AGGACA)GATCCTGCGACCCAGATCTTCTCTCCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613122508_qwi4 AACTGTCTCTGGAGAGAAGATCTGGGTCGCAG(+AGGACA)GATCCCTGGCTGGCACATCATGATCTCTTTC NGG 10 8 17 8 24 0 100 5 10