Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122537_iau9 TGAATGTATGTTTTTCCTGTGTGTATCTGTGG(+AGGACA)GTACCTCATGTGTATGCAGTACCCTTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613122536_emhp GCTGGTTTCCAAGGGTACTGCATACACATGAG(+AGGACA)GTACCCACAGATACACACAGGAAAAACATAC NGG 10 8 17 8 24 0 100 5 10
    240613122534_fzo5 TAGTGTTCTAAGGCAACAGCTTCTCCCCACAG(+AGGACA)GATCCTTCTCCCTCGGCCTTGCTTGGCTACT NGG 10 8 17 8 24 0 100 5 10
    240613122533_wn82 ACACAAGTAGCCAAGCAAGGCCGAGGGAGAAG(+AGGACA)GATCCTGTGGGGAGAAGCTGTTGCCTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613122532_skyi GAAGTCAGAGCCCACTTGTCTCCTGGGTACAG(+AGGACA)GTACCTCATAATGGTCCTGAGATCCAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613122530_x4x5 TGCTGCTCCTTGGATCTCAGGACCATTATGAG(+AGGACA)GTACCTGTACCCAGGAGACAAGTGGGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613122528_yw6b CACAGTGAGACCTTGTCTCAAAAAGAGAGGGG(+AGGACA)GTACCACTAACACCCACCTGGAGGTTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613122527_eblk TTTACTTCAGAACCTCCAGGTGGGTGTTAGTG(+AGGACA)GTACCCCCTCTCTTTTTGAGACAAGGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613122525_tvvc TAATCCCAGCGTTGGGGAAACAGAGCTAGAGG(+AGGACA)GATCCCCATGAGTCCAAGGCTACCCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613122523_c0wb TATATAAACCAGGGTAGCCTTGGACTCATGGG(+AGGACA)GATCCCTCTAGCTCTGTTTCCCCAACGCTGG NGG 10 8 17 8 24 0 100 5 10