Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122507_wene GGGGGGGGGGGCTGGGTTTTTGGAATCTGTAG(+AGGACA)GATCCATAGCACAATCATCTACCACACACCT NGG 10 8 17 8 24 0 100 5 10
    240613122506_mupw GAAGTAGGTGTGTGGTAGATGATTGTGCTATG(+AGGACA)GATCCTACAGATTCCAAAAACCCAGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613122504_ckee CTCCTCTGGGGAGCCTTTCCATAGCAGCTCAG(+AGGACA)GTACCAAAGAGGCAAGGAAAGAGCAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613122503_fqyj CCAGGAACCCCTGCTCTTTCCTTGCCTCTTTG(+AGGACA)GTACCTGAGCTGCTATGGAAAGGCTCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613122502_8v48 GGGAGCCAGCAGAGACCAAAGGGAAGGAGGAG(+AGGACA)GTACCTGAGGCTTGGCAGGATCCCCAGGGGT NGG 10 8 17 8 24 0 100 5 10
    240613122500_1fv5 CAAGGACCCCTGGGGATCCTGCCAAGCCTCAG(+AGGACA)GTACCTCCTCCTTCCCTTTGGTCTCTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613122458_oief GGACCTGGATGTTTCAGGCAATCTGCTGCCTG(+AGGACA)GTACCTATTACTGTCACTATCCCCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240613122457_57pb GGCCACCATGCTGGGGATAGTGACAGTAATAG(+AGGACA)GTACCAGGCAGCAGATTGCCTGAAACATCCA NGG 10 8 17 8 24 0 100 5 10
    240613122455_aaph CATGTGTGCCTGGTGCCAGAGGTCAGAAAAGG(+AGGACA)GTACCACCTCCCCTGGTACTGGAATTTAGGT NGG 10 8 17 8 24 0 100 5 10
    240613122454_8k1a GAACTACCTAAATTCCAGTACCAGGGGAGGTG(+AGGACA)GTACCCTTTTCTGACCTCTGGCACCAGGCAC NGG 10 8 17 8 24 0 100 5 10