Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122403_iucy TCACAACCATCTTTGTAACTCCAGTTTCAAGG(+AGGACA)GATCCAACCCCCTCTCCTGACCTCAAACACC NGG 10 8 17 8 24 0 100 5 10
    240613122402_zfj5 AGTCTGGTGTTTGAGGTCAGGAGAGGGGGTTG(+AGGACA)GATCCCTTGAAACTGGAGTTACAAAGATGGT NGG 10 8 17 8 24 0 100 5 10
    240613122400_x3vw GCATTGGAGTTTCAAATCCCGCAGTCCTGCGG(+AGGACA)GTACCACATTCCTAATGGAGCCTTTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613122358_qtey ATCACAGGCCAAAAGGCTCCATTAGGAATGTG(+AGGACA)GTACCCGCAGGACTGCGGGATTTGAAACTCC NGG 10 8 17 8 24 0 100 5 10
    240613122357_10wb CCCCCTCCCCCAAGCCTGGGCATGGAATGTGG(+AGGACA)GATCCCCAGGCCCTGCCCAGAGCTGGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122354_s3mi GGACCCCTCCCCAGCTCTGGGCAGGGCCTGGG(+AGGACA)GATCCCACATTCCATGCCCAGGCTTGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240613122352_abnw GTCAACTCTCCAGGACAACACTAACCTCTTGG(+AGGACA)GATCCTATGGAGAGAAAAAGGAAGTGTGACA NGG 10 8 17 8 24 0 100 5 10
    240613122350_aur3 ACAGTTGTCACACTTCCTTTTTCTCTCCATAG(+AGGACA)GATCCCAAGAGGTTAGTGTTGTCCTGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613122348_q1a0 TTCTCCTGAGTTTCCCCTGGGTGTAACTCCTG(+AGGACA)GATCCCCTGAGGATCTTCCCACACTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613122346_xcdu GCTAAACCTCCAGTGTGGGAAGATCCTCAGGG(+AGGACA)GATCCAGGAGTTACACCCAGGGGAAACTCAG NGG 10 8 17 8 24 0 100 5 10