Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122330_z8wn ACGGAGAACCAAGGAAGACAATGTTTCATCCG(+AGGACA)GATCCAGAGGCGCATCAAATTCAACAGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613122328_29zc GTGGAGCTGCTGTTGAATTTGATGCGCCTCTG(+AGGACA)GATCCGGATGAAACATTGTCTTCCTTGGTTC NGG 10 8 17 8 24 0 100 5 10
    240613122325_zesa CTCTGGAGTGAAAACATAAAACCTTGTCCCTG(+AGGACA)GTACCGTGAAGCCATGCAAGAGACGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613122324_vo1s TTTTCTCACCTCGTCTCTTGCATGGCTTCACG(+AGGACA)GTACCAGGGACAAGGTTTTATGTTTTCACTC NGG 10 8 17 8 24 0 100 5 10
    240613122322_sg7o ACGTATCCACAGTGCAATGTTGGCTCAGCCAG(+AGGACA)GATCCACCTTGCATACATACTAAAGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240613122320_pj95 GATCAGTCATTCTTTAGTATGTATGCAAGGTG(+AGGACA)GATCCTGGCTGAGCCAACATTGCACTGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613122319_t5nq ATGTAGTGCCTTTTTTGTGTTCTGAGGGAGTG(+AGGACA)GTACCCTGAGGTCTCGTCGTGTAGTTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240613122317_jaqs TTAACTCTAAAACTACACGACGAGACCTCAGG(+AGGACA)GTACCACTCCCTCAGAACACAAAAAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613122315_0h1g TGCCAAGAGCTGGTAAGCGACACAACCCATGG(+AGGACA)GTACCAGGGATTCTGGCTGACACAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613122313_7mvx TCAGGTTCACCTTGTGTCAGCCAGAATCCCTG(+AGGACA)GTACCCATGGGTTGTGTCGCTTACCAGCTCT NGG 10 8 17 8 24 0 100 5 10