Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122224_qrub GCCATTCCGAGCCTGGGACCTGACCCTGTGTG(+AGGACA)GATCCCGTCCCCAGCCAACAGCAGAGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613122222_z7yq GGCCTGGCAGCTCTGCTGTTGGCTGGGGACGG(+AGGACA)GATCCACACAGGGTCAGGTCCCAGGCTCGGA NGG 10 8 17 8 24 0 100 5 10
    240613122221_a4fr AACTGTTCTTGTCCTTGTCACTCAGGGCAGAG(+AGGACA)GATCCTGGGCATACTGAGGAGACCATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613122219_tset GACAATAGCAATGGTCTCCTCAGTATGCCCAG(+AGGACA)GATCCTCTGCCCTGAGTGACAAGGACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613122217_ayt2 AACTGTTCTTGTCCTTGTCACTCAGGGCAGAG(+AGGACA)GATCCTGGGCATACTGAGGAGACCATTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613122215_gusn GACAATAGCAATGGTCTCCTCAGTATGCCCAG(+AGGACA)GATCCTCTGCCCTGAGTGACAAGGACAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613122213_qvjn AAGAATGTGTGTCATGGTAGAGGGGTTCCCAG(+AGGACA)GATCCTCTCCTGCCAGATGCTTGGCTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613122211_zvht GACCTGCAGCAGCCAAGCATCTGGCAGGAGAG(+AGGACA)GATCCTGGGAACCCCTCTACCATGACACACA NGG 10 8 17 8 24 0 100 5 10
    240613122209_gdnq CACCTACCCCGATGCCCGAGTTCAACTCCCAG(+AGGACA)GATCCACTTGGTGGAAGGAGACCACTGATCC NGG 10 8 17 8 24 0 100 5 10
    240613122208_jyoq TGTGGGGATCAGTGGTCTCCTTCCACCAAGTG(+AGGACA)GATCCTGGGAGTTGAACTCGGGCATCGGGGT NGG 10 8 17 8 24 0 100 5 10