Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122150_bgz5 CTGTTGTGTCTAGCTTGTAGGTGTGCTCTGGG(+AGGACA)GATCCCAAGTCAGGTCATCAGACTTGCACTG NGG 10 8 17 8 24 0 100 5 10
    240613122148_lq9i GCTTGCAGTGCAAGTCTGATGACCTGACTTGG(+AGGACA)GATCCCCAGAGCACACCTACAAGCTAGACAC NGG 10 8 17 8 24 0 100 5 10
    240613122146_l6mr CTGGAGTTATAGATGGTTGTGAGTTACCTGTG(+AGGACA)GATCCTGAGAACTGAACCCAAGACTACAGGA NGG 10 8 17 8 24 0 100 5 10
    240613122145_fcjs GCTATTCCTGTAGTCTTGGGTTCAGTTCTCAG(+AGGACA)GATCCACAGGTAACTCACAACCATCTATAAC NGG 10 8 17 8 24 0 100 5 10
    240613122144_cahf TTATTCTGTAGTTGGAGCCCAAACTCATTAAG(+AGGACA)GTACCCTTTATGAGGCCAAGAGAGCCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122142_lzuo ATGCCCCTCTGGCTCTCTTGGCCTCATAAAGG(+AGGACA)GTACCTTAATGAGTTTGGGCTCCAACTACAG NGG 10 8 17 8 24 0 100 5 10
    240613122140_ch20 TGGGGGTTCAGGCTCCAGGATCAATTTAGAGG(+AGGACA)GATCCTTGAACCCAGCCCCGTGAACAATCTA NGG 10 8 17 8 24 0 100 5 10
    240613122139_f5v6 TATGATAGATTGTTCACGGGGCTGGGTTCAAG(+AGGACA)GATCCCTCTAAATTGATCCTGGAGCCTGAAC NGG 10 8 17 8 24 0 100 5 10
    240613122137_f9jk GACTAACATGCCCAGGTGGTGTCGGCTCAGAG(+AGGACA)GATCCTAGCACCATCTTAAGCAGGTGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122136_4d4v CCCTGCCTCCCACCTGCTTAAGATGGTGCTAG(+AGGACA)GATCCTCTGAGCCGACACCACCTGGGCATGT NGG 10 8 17 8 24 0 100 5 10