Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122206_hj7v CCAGTTGGTACACTGTTTTCTGAAAGAGGTGG(+AGGACA)GATCCAAAAGAGCTGTTTCTAGCTGCACTCC NGG 10 8 17 8 24 0 100 5 10
    240613122205_pziz TGCTTGGAGTGCAGCTAGAAACAGCTCTTTTG(+AGGACA)GATCCCACCTCTTTCAGAAAACAGTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240613122203_ir8w TGCCAGTCTGTGTCTGTCTAATCAGAAGGATG(+AGGACA)GATCCCTGTACCTAACATTCCATATCACTAC NGG 10 8 17 8 24 0 100 5 10
    240613122201_6rii TCAGTGTAGTGATATGGAATGTTAGGTACAGG(+AGGACA)GATCCATCCTTCTGATTAGACAGACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240613122200_1ikz AAGGGCTATTCAAGCTCCCAAGAAAATCTGTG(+AGGACA)GATCCATGGGTTACAGATTAAAGTTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240613122158_6hcg ACCCAGTTTGGAACTTTAATCTGTAACCCATG(+AGGACA)GATCCACAGATTTTCTTGGGAGCTTGAATAG NGG 10 8 17 8 24 0 100 5 10
    240613122156_7zde GACATTTTTACCATCTGCTCTCTTCAGTATCG(+AGGACA)GATCCGTATTGAGAAAAGAGAAGCACCAGAC NGG 10 8 17 8 24 0 100 5 10
    240613122155_6oxt GCAGCGTCTGGTGCTTCTCTTTTCTCAATACG(+AGGACA)GATCCGATACTGAAGAGAGCAGATGGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613122153_aycl GGGTCTGAGTTTGCTTTCCGTTGCTGTGGTGG(+AGGACA)GTACCACGACTGGAGCAACATGGGGGAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613122152_6gaj TAAAGCCTTTCCCCCATGTTGCTCCAGTCGTG(+AGGACA)GTACCCACCACAGCAACGGAAAGCAAACTCA NGG 10 8 17 8 24 0 100 5 10