Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122311_cg3g TACTCACGGTCTCTGTTTTCTCACTCTCTGAG(+AGGACA)GTACCACAGGCAGGCAGCCCTTGAGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240613122309_i2m3 CATTGGCTGAGCTCAAGGGCTGCCTGCCTGTG(+AGGACA)GTACCTCAGAGAGTGAGAAAACAGAGACCGT NGG 10 8 17 8 24 0 100 5 10
    240613122308_axis AATAGTGAGCGGGGAAAGGAGAATAGGAAGTG(+AGGACA)GATCCTTCCAGAACCTACCTCTCTCTGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613122307_pyw6 GTTGAGCTTCAGAGAGAGGTAGGTTCTGGAAG(+AGGACA)GATCCACTTCCTATTCTCCTTTCCCCGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613122305_fvvd CCTCTCTTTACGTGAGATACTGTGGCGTGCTG(+AGGACA)GTACCATCCAAGCCTCGGCCCGGTGTCTATC NGG 10 8 17 8 24 0 100 5 10
    240613122304_sko4 GAGGGGATAGACACCGGGCCGAGGCTTGGATG(+AGGACA)GTACCAGCACGCCACAGTATCTCACGTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613122301_18k1 GCACGAGGCAACTGCAGAAGTACACTCCCAGG(+AGGACA)GTACCCTTTTCTCCCTGAAACTTCTTACGGG NGG 10 8 17 8 24 0 100 5 10
    240613122259_3t62 GTGTGCCCGTAAGAAGTTTCAGGGAGAAAAGG(+AGGACA)GTACCCTGGGAGTGTACTTCTGCAGTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240613122258_tnqe TACTGTTCCAGGTGTGGAGGGGGAATGGAGAG(+AGGACA)GTACCTGGTAGGCATCCACAGGGGACATGAT NGG 10 8 17 8 24 0 100 5 10
    240613122256_82ee CTGAGATCATGTCCCCTGTGGATGCCTACCAG(+AGGACA)GTACCTCTCCATTCCCCCTCCACACCTGGAA NGG 10 8 17 8 24 0 100 5 10