Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122344_0zrg GCAAGTCAGCTCTGGATCCCAAAGGGGGCCTG(+AGGACA)GTACCACCCACACCGCTTCTTAGAAGGATCC NGG 10 8 17 8 24 0 100 5 10
    240613122343_8ent TTTCTGGATCCTTCTAAGAAGCGGTGTGGGTG(+AGGACA)GTACCAGGCCCCCTTTGGGATCCAGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613122341_9fmr CAGAACCCAGACTCTTACCCTTACCCAAAATG(+AGGACA)GATCCAGTTCCACTGCCTCCCTCTCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613122340_f23m CAGTGAGGAGGGAGAGGGAGGCAGTGGAACTG(+AGGACA)GATCCATTTTGGGTAAGGGTAAGAGTCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613122338_4wg1 TATCCTTTCTGTTTTGGGCTCTACAGCCTCTG(+AGGACA)GATCCACTGGGCCTTGGCAACCCTTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613122336_01dk GAGCCAAGAACAAGGGTTGCCAAGGCCCAGTG(+AGGACA)GATCCAGAGGCTGTAGAGCCCAAAACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613122335_7paw CCTCCAGAGCTCACCTTCCACGCAGTGATGAG(+AGGACA)GATCCTGTCAGGGACCCTACCTCACAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613122334_gvn0 AGCTGACTGCTGTGAGGTAGGGTCCCTGACAG(+AGGACA)GATCCTCATCACTGCGTGGAAGGTGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613122332_bw0e GCAGGGTGGCAGTCACCTCCCCTGTCCACGTG(+AGGACA)GATCCATCCCTGCCATTGTGTGAGTCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613122331_51dj CTATCCCTCTGACTCACACAATGGCAGGGATG(+AGGACA)GATCCACGTGGACAGGGGAGGTGACTGCCAC NGG 10 8 17 8 24 0 100 5 10