Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122238_e1xw GCATGTACATCTGTGTACCATGCGTGTGTGTG(+AGGACA)GTACCAGCAGAAGTCAGCAGAGGGCGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613122237_s6er AGGAACTCAGCGCCCTCTGCTGACTTCTGCTG(+AGGACA)GTACCACACACACGCATGGTACACAGATGTA NGG 10 8 17 8 24 0 100 5 10
    240613122235_q3yq CACAGCACGTGTCAAGCTTGTATGAGACGCAG(+AGGACA)GATCCTATCACCAGCACTGAGAATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240613122234_0iau ACTTGAAGTTTTATTCTCAGTGCTGGTGATAG(+AGGACA)GATCCTGCGTCTCATACAAGCTTGACACGTG NGG 10 8 17 8 24 0 100 5 10
    240613122233_78ks GACACATCCTGGTGTGTCCACATGTGTGCATG(+AGGACA)GATCCATGTGTGCGCATTGGGGTGGGGGTGG NGG 10 8 17 8 24 0 100 5 10
    240613122232_rrql AGAGCCCACCCCCACCCCAATGCGCACACATG(+AGGACA)GATCCATGCACACATGTGGACACACCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240613122230_2m77 CCTTAAGTCCCTGTTCTCTGAAGCAAGGGCAG(+AGGACA)GATCCCTACCCAAGAGTTGCTGAGGCCCAAG NGG 10 8 17 8 24 0 100 5 10
    240613122229_223o AACTGCTTGGGCCTCAGCAACTCTTGGGTAGG(+AGGACA)GATCCTGCCCTTGCTTCAGAGAACAGGGACT NGG 10 8 17 8 24 0 100 5 10
    240613122226_0056 CAAACAGAACCTACTCACAAGACATCCCCAAG(+AGGACA)GATCCAGACATGCAGTCCCAGAGAGAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613122225_3s7w TATGTGTCCTTCTCTCTGGGACTGCATGTCTG(+AGGACA)GATCCTTGGGGATGTCTTGTGAGTAGGTTCT NGG 10 8 17 8 24 0 100 5 10