Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122059_u8c1 CATTGTGCCTCTGAGACATCCTTAGGGCTGGG(+AGGACA)GTACCCAGCTCTCGAAGACACCATGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240613122057_f2c4 AGAAGTCATGGCATGGTGTCTTCGAGAGCTGG(+AGGACA)GTACCCCAGCCCTAAGGATGTCTCAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613122056_ari8 ACTAACATCTCCCTCTCCCTCTCTTGCTATGG(+AGGACA)GATCCCAATGGTACTGCAGGCAACACAGTAC NGG 10 8 17 8 24 0 100 5 10
    240613122054_pvxi GACTGGTACTGTGTTGCCTGCAGTACCATTGG(+AGGACA)GATCCCATAGCAAGAGAGGGAGAGGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240613122051_gl4a GGCTCTTCTGGCTTTTACCTTCACCGATGGGG(+AGGACA)GTACCTGGTGTCCTGAGGTGGCCAAGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613122050_n1bd TGTAGGCTCACTTGGCCACCTCAGGACACCAG(+AGGACA)GTACCCCCATCGGTGAAGGTAAAAGCCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613122048_f8pv CAGGTGGATGTTTGGGTTCTGTTGGTAACTGG(+AGGACA)GTACCTGCAAAAGAGCTTAGGACACTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613122047_fp5i TAATGAGCAAAGTGTCCTAAGCTCTTTTGCAG(+AGGACA)GTACCCAGTTACCAACAGAACCCAAACATCC NGG 10 8 17 8 24 0 100 5 10
    240613122046_llmj TCCAGCATGAGCAGGCTCCCTAGCTGTTGAAG(+AGGACA)GATCCCATGGGTCAGGCACTGACCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613122043_a18c CACCTTCAAGGTGGTCAGTGCCTGACCCATGG(+AGGACA)GATCCTTCAACAGCTAGGGAGCCTGCTCATG NGG 10 8 17 8 24 0 100 5 10