Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121958_sws7 ACTCCATAGATCTCAATAATTCTGGAAACAAG(+AGGACA)GTACCATGACATGCAGGTCATCATCGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613121957_u8wm TTTGGTTTTCCGATGATGACCTGCATGTCATG(+AGGACA)GTACCTTGTTTCCAGAATTATTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240613121955_ei9b AACAAAAAACAAAAAACACTGGGATAAAGGAG(+AGGACA)GTACCCAAGAATCAGTGGAGTTTACCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240613121954_3d9b TCACAGCTAGGGTAAACTCCACTGATTCTTGG(+AGGACA)GTACCTCCTTTATCCCAGTGTTTTTTGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613121953_8khw CAGGAATAGAAATCCTGACCAAGACATTGTTG(+AGGACA)GTACCAGCATAGTGGGGCATTCCTGTGACAA NGG 10 8 17 8 24 0 100 5 10
    240613121951_n3e8 TCAGGTTGTCACAGGAATGCCCCACTATGCTG(+AGGACA)GTACCAACAATGTCTTGGTCAGGATTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613121950_jes3 AGTGATGAAAAGGAGAGGGCCCCTTGTGGGTG(+AGGACA)GTACCATCTCTGGGCTGGTAATATTGGATTC NGG 10 8 17 8 24 0 100 5 10
    240613121948_amrk TTATAGAATCCAATATTACCAGCCCAGAGATG(+AGGACA)GTACCACCCACAAGGGGCCCTCTCCTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613121947_wfwh CCTTCTCCCCACTGTAGGAGATTTCATCAAAG(+AGGACA)GTACCTTCCTTTGGGTCCTGAGAACCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613121946_15bn CTCTTTGGGAGGTTCTCAGGACCCAAAGGAAG(+AGGACA)GTACCTTTGATGAAATCTCCTACAGTGGGGA NGG 10 8 17 8 24 0 100 5 10