Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122027_67b9 CAAGTCTGTGCCTCAGCACAGCTAGAAGTCAG(+AGGACA)GATCCAAGGAGGCCTAAAATCGGCAATGACA NGG 10 8 17 8 24 0 100 5 10
    240613122025_dqhq GAGTCTGTCATTGCCGATTTTAGGCCTCCTTG(+AGGACA)GATCCTGACTTCTAGCTGTGCTGAGGCACAG NGG 10 8 17 8 24 0 100 5 10
    240613122024_iykt CTCCCCATGGATTCATGTGCACCCATCTGGAG(+AGGACA)GTACCAGATTCCTCCATGGCAGATCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613122022_1r77 TGTTCCCAACAGATCTGCCATGGAGGAATCTG(+AGGACA)GTACCTCCAGATGGGTGCACATGAATCCATG NGG 10 8 17 8 24 0 100 5 10
    240613122021_mzwv CCCCATGCTACTGATAACACAGTGATCACCAG(+AGGACA)GATCCAAACTGGCTTCAGGTTTCTAACAAAA NGG 10 8 17 8 24 0 100 5 10
    240613122019_y4d5 GACGCTTTTGTTAGAAACCTGAAGCCAGTTTG(+AGGACA)GATCCTGGTGATCACTGTGTTATCAGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240613122018_q5wt ACTCCATAGATCTCAGTAATTCTGGAAACAAG(+AGGACA)GTACCATGACATGCAGGTCATCATTGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613122017_g7h1 TTTGGTTTTCCAATGATGACCTGCATGTCATG(+AGGACA)GTACCTTGTTTCCAGAATTACTGAGATCTAT NGG 10 8 17 8 24 0 100 5 10
    240613122016_f27s AAAAGGATATACCTTCTTCTCAGCACCTCATG(+AGGACA)GTACCTTCTCCAAAATTGACCACATAATAGG NGG 10 8 17 8 24 0 100 5 10
    240613122015_7xgk TGTGACCTATTATGTGGTCAATTTTGGAGAAG(+AGGACA)GTACCATGAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10