Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122117_md27 AGGACGCTAGAGGGCGCTCTAGGACAGCGTCG(+AGGACA)GATCCTGTCTCTGGCAACCCCTTGCAAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613122116_8ofo CCCGTGGGCTTGCAAGGGGTTGCCAGAGACAG(+AGGACA)GATCCGACGCTGTCCTAGAGCGCCCTCTAGC NGG 10 8 17 8 24 0 100 5 10
    240613122114_pq8y GGCATCTGACTCAAGGAATAGTCTGGAATAAG(+AGGACA)GATCCGGGTCTGTTTACCCTGGTAGCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240613122112_75cg TCAGCCGGAGGCTACCAGGGTAAACAGACCCG(+AGGACA)GATCCTTATTCCAGACTATTCCTTGAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613122109_bf2b AAGCCATCCTAGGAAGACCAAGTGATCCCATG(+AGGACA)GATCCTGTACACCTCTCCTGGAGGAGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613122108_u6v8 TGTACCTGGGCTCCTCCAGGAGAGGTGTACAG(+AGGACA)GATCCATGGGATCACTTGGTCTTCCTAGGAT NGG 10 8 17 8 24 0 100 5 10
    240613122107_ejjz TGCTGAGACACAGAATGAGTTTTATGGCAAAG(+AGGACA)GATCCTTAATCCCCAGAGACGCTTTGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613122105_g4my CACCACAAGCCAAAGCGTCTCTGGGGATTAAG(+AGGACA)GATCCTTTGCCATAAAACTCATTCTGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613122103_zpuq TTTCTCTGGAGTCCTTCTTGGAGAAAACCAGG(+AGGACA)GATCCAGGTTCATCGCCGAGGCCCTCGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613122101_8or2 GCCCCGCTCCGAGGGCCTCGGCGATGAACCTG(+AGGACA)GATCCCTGGTTTTCTCCAAGAAGGACTCCAG NGG 10 8 17 8 24 0 100 5 10