Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121913_1jey TGGATTAGCTGCCAATCTCAGTTATTGCCAGG(+AGGACA)GATCCTGTTCCAAACACCCAAACTTCAACTG NGG 10 8 17 8 24 0 100 5 10
    240613121912_742y AAGCTCCATTTCCAATACTCCTAGGGGTACAG(+AGGACA)GATCCACTCTGTGGGACCCACAACAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613121911_vxxc ATTCCCTTCCCTGTTGTGGGTCCCACAGAGTG(+AGGACA)GATCCTGTACCCCTAGGAGTATTGGAAATGG NGG 10 8 17 8 24 0 100 5 10
    240613121909_4ck0 GCACATGAGTATGGTAGACACTAGCCTTCCAG(+AGGACA)GATCCCTATACTCCTAGTACCTTGCATTATT NGG 10 8 17 8 24 0 100 5 10
    240613121908_3hu0 ACAAAAATAATGCAAGGTACTAGGAGTATAGG(+AGGACA)GATCCTGGAAGGCTAGTGTCTACCATACTCA NGG 10 8 17 8 24 0 100 5 10
    240613121906_mbu8 TTAATTTTTGACAGTGTTCAAAATGTGCAGAG(+AGGACA)GATCCTTTGTCCAACCCTTTGACATGACAAT NGG 10 8 17 8 24 0 100 5 10
    240613121905_uu6c GTCCTATTGTCATGTCAAAGGGTTGGACAAAG(+AGGACA)GATCCTCTGCACATTTTGAACACTGTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613121904_antx ATGAGCCCCGAGGGTCTGCCTGCGACTCGCAG(+AGGACA)GATCCGCCAAGAAACTGCAAGAACGGATGGG NGG 10 8 17 8 24 0 100 5 10
    240613121902_cf6i TTTCCCCCATCCGTTCTTGCAGTTTCTTGGCG(+AGGACA)GATCCTGCGAGTCGCAGGCAGACCCTCGGGG NGG 10 8 17 8 24 0 100 5 10
    240613121901_albw AAAAAAAAAAAGACTTTGAAAAGAAAGATCTG(+AGGACA)GTACCAGGGCAGGCTTGGTGCAATGCCCACG NGG 10 8 17 8 24 0 100 5 10