Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121829_j4ml ACTGTAACTATCCAGAGACTGCCCCATCCGGG(+AGGACA)GATCCATCCCATAATCAGCCACCAAACGCAG NGG 10 8 17 8 24 0 100 5 10
    240613121827_f7c6 AAGCATGGACCAACTTAAAGGGTTTGCACTGG(+AGGACA)GATCCTCTGTGGAGCTGAGTTCTACCACCTT NGG 10 8 17 8 24 0 100 5 10
    240613121826_crx7 GTCAGAAGGTGGTAGAACTCAGCTCCACAGAG(+AGGACA)GATCCCAGTGCAAACCCTTTAAGTTGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240613121823_0oaq GCAGACAGTGTATCTGAAAAGTCTCTTCCCAG(+AGGACA)GATCCAGAATGGGATGAACCTGTCTTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613121822_q2f4 GAGGGAGCACAAGACAGGTTCATCCCATTCTG(+AGGACA)GATCCTGGGAAGAGACTTTTCAGATACACTG NGG 10 8 17 8 24 0 100 5 10
    240613121820_wors AAGTGTTGTTGGGCAGTCCCGTGTCATCCATG(+AGGACA)GTACCTGCTTCAATTTCACACATAGCTGGTA NGG 10 8 17 8 24 0 100 5 10
    240613121819_nf9s CAGTTTACCAGCTATGTGTGAAATTGAAGCAG(+AGGACA)GTACCATGGATGACACGGGACTGCCCAACAA NGG 10 8 17 8 24 0 100 5 10
    240613121818_u113 AAAGTTGTAGAAAACTTTTATTTCTTCAAGAG(+AGGACA)GTACCAACTATGGTTGATACAATGTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613121816_erik TCAGTTCAGACACATTGTATCAACCATAGTTG(+AGGACA)GTACCTCTTGAAGAAATAAAAGTTTTCTACA NGG 10 8 17 8 24 0 100 5 10
    240613121815_14ed AGTAAAAGCAGTCACTTCACTGGGCATTGCTG(+AGGACA)GTACCGGCAGGGGGTGTGTAGGTTAGTTCTG NGG 10 8 17 8 24 0 100 5 10