Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121813_8dsm TGTGTCAGAACTAACCTACACACCCCCTGCCG(+AGGACA)GTACCAGCAATGCCCAGTGAAGTGACTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613121812_psn0 CTCCGTTACGATATATCCTAGCCACATGCAGG(+AGGACA)GTACCACATAGGATTTTCTATATTGAAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613121810_c51f AAGTATTCTTTCAATATAGAAAATCCTATGTG(+AGGACA)GTACCCTGCATGTGGCTAGGATATATCGTAA NGG 10 8 17 8 24 0 100 5 10
    240613121809_m5rc CCTGGGCCTGTTGGAGTGTAGCTATGTTGGTG(+AGGACA)GATCCTGTATACAGGTGAAAAAGTGCCATTG NGG 10 8 17 8 24 0 100 5 10
    240613121808_1eiw AACGACAATGGCACTTTTTCACCTGTATACAG(+AGGACA)GATCCACCAACATAGCTACACTCCAACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613121806_s2ch AAATCAGGAATCTGACTCTTGCTAGGCTACCG(+AGGACA)GATCCCTGCATGCTCCTCTGAGTTTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613121804_2dm7 TCATGCTGCCCAAACTCAGAGGAGCATGCAGG(+AGGACA)GATCCGGTAGCCTAGCAAGAGTCAGATTCCT NGG 10 8 17 8 24 0 100 5 10
    240613121803_2gau CAAAAAATATCTGACAATAAAATAGCCCACTG(+AGGACA)GATCCTGTTCAGGCTGGCTGATATCTTGATT NGG 10 8 17 8 24 0 100 5 10
    240613121802_mmcy TGTTGAATCAAGATATCAGCCAGCCTGAACAG(+AGGACA)GATCCAGTGGGCTATTTTATTGTCAGATATT NGG 10 8 17 8 24 0 100 5 10
    240613121800_9efr CACATGGAACCAGGGTATGGGACCTTCACACG(+AGGACA)GATCCTTCTGGAAGTCTCCCTTTGGTGTGGA NGG 10 8 17 8 24 0 100 5 10