Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121743_lfw4 GGCCTCAGCCCCTGGCCGTGTGGAATCCATGG(+AGGACA)GATCCTCGGGACTCCCCAGACAGGCGTGTGT NGG 10 8 17 8 24 0 100 5 10
    240613121741_pali CTGACCTTGGCTTGCCAATCGGTGAACTGAGG(+AGGACA)GATCCCTTCAGAGCAGGTCCCAGCCAACCCA NGG 10 8 17 8 24 0 100 5 10
    240613121739_3xck ATTGTTGGGTTGGCTGGGACCTGCTCTGAAGG(+AGGACA)GATCCCTCAGTTCACCGATTGGCAAGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240613121738_3pmw GAAAGTTTTTAAGCCACCAGCTGAGATGAGAG(+AGGACA)GTACCTTTAAGCCTAGCCCACCATCACATCG NGG 10 8 17 8 24 0 100 5 10
    240613121736_viy0 CTCACCGATGTGATGGTGGGCTAGGCTTAAAG(+AGGACA)GTACCTCTCATCTCAGCTGGTGGCTTAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613121735_2rmb CCTCAGGGTTAGTAAGGCAGGAGCTGCTAGTG(+AGGACA)GTACCACAGGAACAGCAATGGCTGACATTAA NGG 10 8 17 8 24 0 100 5 10
    240613121733_loo8 TGAAGTTAATGTCAGCCATTGCTGTTCCTGTG(+AGGACA)GTACCACTAGCAGCTCCTGCCTTACTAACCC NGG 10 8 17 8 24 0 100 5 10
    240613121731_b2l2 GAGGTGGGGTGCTTTATAGGATTCTGCCTTCG(+AGGACA)GATCCAAAACTCTGACCAGGGTGGCTGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613121729_gglh GAGATGGAGCAGCCACCCTGGTCAGAGTTTTG(+AGGACA)GATCCGAAGGCAGAATCCTATAAAGCACCCC NGG 10 8 17 8 24 0 100 5 10
    240613121727_n74x CATCTCTATAACCGTTTGCTCTTGGACTCCCG(+AGGACA)GATCCCTGTACCCCAGGTTGTTTTCCCTGTA NGG 10 8 17 8 24 0 100 5 10