Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121636_0y8y TTCAAATTCCCAGAATCCTGAGGACCAAGAAG(+AGGACA)GATCCCTCAGCTGCTACTTCCAGCACCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613121634_q5al TCAAGGACGCAACCTGGGGTTGCCAAGACCAG(+AGGACA)GATCCAGACCCACGAGTGATGGGCCGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613121633_66c4 TGTTGTGCCTCGGCCCATCACTCGTGGGTCTG(+AGGACA)GATCCTGGTCTTGGCAACCCCAGGTTGCGTC NGG 10 8 17 8 24 0 100 5 10
    240613121631_qzyl AGTGTAGCATGCGGTCGGCATTGAGAACCTTG(+AGGACA)GTACCAAAGGCCCCTGAGTCTTCCACTTACT NGG 10 8 17 8 24 0 100 5 10
    240613121630_xznl AAGAGAGTAAGTGGAAGACTCAGGGGCCTTTG(+AGGACA)GTACCAAGGTTCTCAATGCCGACCGCATGCT NGG 10 8 17 8 24 0 100 5 10
    240613121629_9vj6 TACACACTAATCAGTTTTGTGCTGACAGAAAG(+AGGACA)GATCCTAGTTAAAATACTGATTCTCAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613121628_4isc ATGTTACTTTTGAGAATCAGTATTTTAACTAG(+AGGACA)GATCCTTTCTGTCAGCACAAAACTGATTAGT NGG 10 8 17 8 24 0 100 5 10
    240613121626_jzpj ACCCTGAGATCCTTGTGTGAGCAAGATCCTGG(+AGGACA)GATCCTCTGATCCTCTGATCCTGGGCGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613121625_641r ACTCTAACACGCCCAGGATCAGAGGATCAGAG(+AGGACA)GATCCCAGGATCTTGCTCACACAAGGATCTC NGG 10 8 17 8 24 0 100 5 10
    240613121624_0oxo CATGATTTAATGTTGACACCTTGGTCTAGAAG(+AGGACA)GATCCACTCGGGGTTGCTAGGCTCCCTTACA NGG 10 8 17 8 24 0 100 5 10