Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121709_84kp CAAAAAGTTGTTCTTGTAGCCTCTGGCTCTGG(+AGGACA)GATCCTGGGAAAATTTTGGTTGTTTGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240613121707_qmu8 GCTTCTCAGCCCCACCCTCACCCTCTGGCCAG(+AGGACA)GATCCTACAAGGCTACATCCCCTGCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613121706_mrg8 TCAACCAAGCAGCAGGGGATGTAGCCTTGTAG(+AGGACA)GATCCTGGCCAGAGGGTGAGGGTGGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613121704_y2e0 CAGGCTGCTGCCCCTCTGCAGCTCTACCAGAG(+AGGACA)GTACCCGGCCTACTCTGCTGGAAAACTAGGT NGG 10 8 17 8 24 0 100 5 10
    240613121702_rrdq CCCAGACCTAGTTTTCCAGCAGAGTAGGCCGG(+AGGACA)GTACCTCTGGTAGAGCTGCAGAGGGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613121700_n6ok CAGTCTCCTCTACCCTCTGAACCACCTCCTCG(+AGGACA)GATCCCATAGGCTACATTTTTTTTCCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613121658_c8ku AATTTTGGAAGGAAAAAAAATGTAGCCTATGG(+AGGACA)GATCCGAGGAGGTGGTTCAGAGGGTAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613121655_wxhw CATGTCATGCTCTTCAGTGTAGGGCTTCCCGG(+AGGACA)GTACCGTGGTGCTTGAATATTATGCCATCTC NGG 10 8 17 8 24 0 100 5 10
    240613121654_ha6g GGTGGGAGATGGCATAATATTCAAGCACCACG(+AGGACA)GTACCCGGGAAGCCCTACACTGAAGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240613121652_f2gz AAGCTTCCAAGTGCTGGGGAAAGTCCAGCTAG(+AGGACA)GATCCCATTGGTTCCTTGGCTTGATGTGCAA NGG 10 8 17 8 24 0 100 5 10