Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121845_yd1o AGAGAGGAAGTGAAGATTAGCTGAATAGTTGG(+AGGACA)GTACCAGTGCAGAGAGCAGAGAGCTTCTTGT NGG 10 8 17 8 24 0 100 5 10
    240613121844_t1fp AGTGTAGGGGCTTTAGCATCTTGATTTTTCAG(+AGGACA)GATCCGATTCTGCCCAATGGTAATTTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240613121843_m3lh TCCTTTTAGCAAATTACCATTGGGCAGAATCG(+AGGACA)GATCCTGAAAAATCAAGATGCTAAAGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613121841_u0cc GGTGTCAGCGTGTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613121838_oxfl GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCACACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613121837_9q3q TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AGGACA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613121835_qrxc TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AGGACA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613121834_pfcv CTTGTACTTTTCTTTATTCTCTACAAGACTGG(+AGGACA)GATCCATCTTGGAAACATTAAGAATATCTAC NGG 10 8 17 8 24 0 100 5 10
    240613121833_pec0 ATCCAGTAGATATTCTTAATGTTTCCAAGATG(+AGGACA)GATCCCAGTCTTGTAGAGAATAAAGAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613121831_p4lm AATGTCTGCGTTTGGTGGCTGATTATGGGATG(+AGGACA)GATCCCCGGATGGGGCAGTCTCTGGATAGTT NGG 10 8 17 8 24 0 100 5 10