Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121859_4vgh ACGTACGTGGGCATTGCACCAAGCCTGCCCTG(+AGGACA)GTACCAGATCTTTCTTTTCAAAGTCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613121857_g9mq GGTGTTGCACTCCAACACTTTTTACTATTCCG(+AGGACA)GATCCCAGCAAAGGTACACATCAGCAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613121855_o93p ATTGATTTCCTGCTGATGTGTACCTTTGCTGG(+AGGACA)GATCCGGAATAGTAAAAAGTGTTGGAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240613121854_fbdq CCTGACTTCCAGTTAAGATGGAAGATTAGAAG(+AGGACA)GTACCTCTCCTTGTGACCCACTTAAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613121853_8e79 GCAGTTTCACCTTAAGTGGGTCACAAGGAGAG(+AGGACA)GTACCTTCTAATCTTCCATCTTAACTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613121852_g02n AGCAAGGAAGGGCTAGCCACCATCTTGAGAAG(+AGGACA)GTACCTGTTGTTCAAAACTAACTCACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613121851_814r ACCCCCCAGAGTGAGTTAGTTTTGAACAACAG(+AGGACA)GTACCTTCTCAAGATGGTGGCTAGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613121850_xffn TTTCCCTCAGTGATGAAGAGGCCCCACTGTGG(+AGGACA)GTACCAACCAACCCTGATACATAAAGTCACA NGG 10 8 17 8 24 0 100 5 10
    240613121848_awma TTCCCTGTGACTTTATGTATCAGGGTTGGTTG(+AGGACA)GTACCCACAGTGGGGCCTCTTCATCACTGAG NGG 10 8 17 8 24 0 100 5 10
    240613121847_r8fy ACTTCACAAGAAGCTCTCTGCTCTCTGCACTG(+AGGACA)GTACCCAACTATTCAGCTAATCTTCACTTCC NGG 10 8 17 8 24 0 100 5 10