Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613122623_dddn CCAGCTATCCTGCTTCCCAGGGCCACCAACTG(+AGGACA)GATCCAAGGACCAGGGTGTGTGCAGGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613122622_cvgp TTTGCTGGAGCCTGCACACACCCTGGTCCTTG(+AGGACA)GATCCAGTTGGTGGCCCTGGGAAGCAGGATA NGG 10 8 17 8 24 0 100 5 10
    240613122620_g04b GCTCATAGAACACGGCGCATGATGGGTAAGAG(+AGGACA)GATCCAGACTACCCAGTGTGTCCCAGGTATT NGG 10 8 17 8 24 0 100 5 10
    240613122619_li68 CTGGGAATACCTGGGACACACTGGGTAGTCTG(+AGGACA)GATCCTCTTACCCATCATGCGCCGTGTTCTA NGG 10 8 17 8 24 0 100 5 10
    240613122618_r4zl TTAGTGTCCTTTAGTCTTCGCACTTGTCTAAG(+AGGACA)GATCCACTTTTAGGGCTTGCCCACATGTGAT NGG 10 8 17 8 24 0 100 5 10
    240613122617_y0xx ATAGAATCACATGTGGGCAAGCCCTAAAAGTG(+AGGACA)GATCCTTAGACAAGTGCGAAGACTAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613122616_up2r GCTGTCTTCAGACCCATTAGAAGAGGGCATCG(+AGGACA)GATCCCATTATAGATGGTTATAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613122614_lfba ACCACATGGTGGCTTATAACCATCTATAATGG(+AGGACA)GATCCGATGCCCTCTTCTAATGGGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613122611_og35 GGTCAGGAGGACCCAGCGCCTACTGCACCCAG(+AGGACA)GATCCATTAGCACTGTAACAACGAACACCTG NGG 10 8 17 8 24 0 100 5 10
    240613122610_loph CCACTCAGGTGTTCGTTGTTACAGTGCTAATG(+AGGACA)GATCCTGGGTGCAGTAGGCGCTGGGTCCTCC NGG 10 8 17 8 24 0 100 5 10