Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123125_od06 AAACACCTGTTAGTTCTAACTGTGTAGATTGG(+AGGACA)GTACCATAGTTAAGCTTAAGGATCCGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240613123124_o7oz CGTTCACACCCGGATCCTTAAGCTTAACTATG(+AGGACA)GTACCCAATCTACACAGTTAGAACTAACAGG NGG 10 8 17 8 24 0 100 5 10
    240613123123_knao TGTGTAGATTGGGTACCATAGTTAAGCTTAAG(+AGGACA)GATCCGGGTGTGAACGGCACACAAGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613123121_x7vb AGAAGCCCCAGCTTGTGTGCCGTTCACACCCG(+AGGACA)GATCCTTAAGCTTAACTATGGTACCCAATCT NGG 10 8 17 8 24 0 100 5 10
    240613123120_vsdi GCGAGGGGACACAGCAACCGTGCAAACGTCAG(+AGGACA)GATCCTGCATTCTTTAGATTGTTTCCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613123118_li8m TAATTTAAAAGGAAACAATCTAAAGAATGCAG(+AGGACA)GATCCTGACGTTTGCACGGTTGCTGTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240613123117_qeta TAAAGTATTTGCCATGCAAGCAGGAAGACCTG(+AGGACA)GATCCTCATAGAAAGAAGGCAGGCAACATCA NGG 10 8 17 8 24 0 100 5 10
    240613123115_8siv CCATCTGATGTTGCCTGCCTTCTTTCTATGAG(+AGGACA)GATCCAGGTCTTCCTGCTTGCATGGCAAATA NGG 10 8 17 8 24 0 100 5 10
    240613123114_mg8d ATGAGAAAGAAACACGGCAACACACTCTATTG(+AGGACA)GTACCTGTGTAAGTTAAAGTTCAGGGTCGAC NGG 10 8 17 8 24 0 100 5 10
    240613123113_6vu8 GTTCAGTCGACCCTGAACTTTAACTTACACAG(+AGGACA)GTACCAATAGAGTGTGTTGCCGTGTTTCTTT NGG 10 8 17 8 24 0 100 5 10