Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123210_09jm TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+AGGACA)GATCCTCCTGTAGTACTGTGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613123209_mcmq TACTCAGAAAATAGTTCACAGTACTACAGGAG(+AGGACA)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240613123208_fkt9 CACAATTTTCCTTTTCATCCCCCACCACATTG(+AGGACA)GATCCTAAATATATACCATCACTTTTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240613123206_jwwo CCTCTACGAGAAAAGTGATGGTATATATTTAG(+AGGACA)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613123205_68tg ACTGTCTGGGTTTGGTGACTGATGATGGAATG(+AGGACA)GATCCCCAGGTAGAGCAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613123203_z259 GGAATGACCATCCAGAGACTGCTCTACCTGGG(+AGGACA)GATCCATTCCATCATCAGTCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613123201_us4d TTCTGGGTATGTGTCCAGGAGAATAATTGCTG(+AGGACA)GATCCTCCTGTAGTACTATGAACTATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613123200_71ie TACTCAGAAAATAGTTCATAGTACTACAGGAG(+AGGACA)GATCCAGCAATTATTCTCCTGGACACATACC NGG 10 8 17 8 24 0 100 5 10
    240613123159_4yro CACAATTTTCCTTTTCATCCCCCACCACATTG(+AGGACA)GATCCTAAATATATACCATCACTTTTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240613123157_1h89 CCTCTATGAGAAAAGTGATGGTATATATTTAG(+AGGACA)GATCCAATGTGGTGGGGGATGAAAAGGAAAA NGG 10 8 17 8 24 0 100 5 10