Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123339_mbtr CCTAGAACACATAGGACCCGAAGATTCCTGAG(+AGGACA)GATCCGAGAAGATTCCCGAGGACTGAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613123338_zvjl CGGCGTCTCCTCAGTCCTCGGGAATCTTCTCG(+AGGACA)GATCCTCAGGAATCTTCGGGTCCTATGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613123336_hlja GGATTGAACTCAAATTGCTAGGTCGGCAGCAG(+AGGACA)GTACCTTTACCCACTGGACCATCTAGTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613123335_oah3 AAGGTGACAACTAGATGGTCCAGTGGGTAAAG(+AGGACA)GTACCTGCTGCCGACCTAGCAATTTGAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613123333_1tqy ACAAGAAGATACCTGAGAGAAGCCATGGGGAG(+AGGACA)GATCCAGCATTGATGGTGTAGCAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613123332_qhct GGCCTCTGGCTTCTGCTACACCATCAATGCTG(+AGGACA)GATCCTCCCCATGGCTTCTCTCAGGTATCTT NGG 10 8 17 8 24 0 100 5 10
    240613123331_sjeo GTTCTCAAAACAAATGTTCTTAGTTCTCAGTG(+AGGACA)GATCCTGCAGATCTTAAATCATTCTTTGCGT NGG 10 8 17 8 24 0 100 5 10
    240613123330_jfdq TAAAAACGCAAAGAATGATTTAAGATCTGCAG(+AGGACA)GATCCACTGAGAACTAAGAACATTTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613123328_jt15 GCAGTCTTTTTAGCAGTAGACTCAGAAGTCAG(+AGGACA)GATCCTCAGACCAACTCAATATATACTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613123327_wgh7 AAAACTAAAAGTATATATTGAGTTGGTCTGAG(+AGGACA)GATCCTGACTTCTGAGTCTACTGCTAAAAAG NGG 10 8 17 8 24 0 100 5 10