Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123443_2c0q GTGATTTAATCATGATTGTAAAAATTGTGTGG(+AGGACA)GTACCTTAAAAAAAAACTGCTTCTGTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613123442_3bef GCGACCAAAGACAGAAGCAGTTTTTTTTTAAG(+AGGACA)GTACCCACACAATTTTTACAATCATGATTAA NGG 10 8 17 8 24 0 100 5 10
    240613123440_df8l TCCAGGGTGTCAAGAGAGCTGGGCCTGACCTG(+AGGACA)GTACCTTTGCCCCGTGTTGCTGCTATGCTGC NGG 10 8 17 8 24 0 100 5 10
    240613123439_xx2h CCGCTGCAGCATAGCAGCAACACGGGGCAAAG(+AGGACA)GTACCAGGTCAGGCCCAGCTCTCTTGACACC NGG 10 8 17 8 24 0 100 5 10
    240613123437_osgy CAGACCTGCTCAGACAGGAAGTAAAGCCAGAG(+AGGACA)GTACCAGGGTGAGGAGCTGTGTGCCTCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613123434_hzkj CTCTTTGGAGAGGCACACAGCTCCTCACCCTG(+AGGACA)GTACCTCTGGCTTTACTTCCTGTCTGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613123433_qg6j CACAAATATCTTCAATTTCCAGGTACAGGGAG(+AGGACA)GTACCTTGATAGATTCCCGCCCTGAGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613123431_mjbb CAATGCAAGCCTCAGGGCGGGAATCTATCAAG(+AGGACA)GTACCTCCCTGTACCTGGAAATTGAAGATAT NGG 10 8 17 8 24 0 100 5 10
    240613123430_tick ACTGTTTTTCAAAACAATTTATTGCATATTTG(+AGGACA)GTACCTTATGTTTGACAACAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613123429_kz0g AATTTCTTTTTTTTTTTGTTGTCAAACATAAG(+AGGACA)GTACCAAATATGCAATAAATTGTTTTGAAAA NGG 10 8 17 8 24 0 100 5 10