Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123514_m7i7 TTCGTGCACACAGTGGCCAGAAAAGGGTATTG(+AGGACA)GATCCCTTGGAGCTAGACTTACAAAGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240613123511_fnki GCTTACACCCCTTTGTAAGTCTAGCTCCAAGG(+AGGACA)GATCCAATACCCTTTTCTGGCCACTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613123510_2lfn TTTCTTTGTTCCCCTTCTGTAAGCTCTCTTAG(+AGGACA)GATCCACATATAGAAGAAACACATTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240613123509_j0ui AGATTTAAATTAATGTGTTTCTTCTATATGTG(+AGGACA)GATCCTAAGAGAGCTTACAGAAGGGGAACAA NGG 10 8 17 8 24 0 100 5 10
    240613123507_aow7 CACCTTGTTATTTATTTATTTTAATGTAAAAG(+AGGACA)GATCCCTTAACCCACTGCTTGGCAGAATTAC NGG 10 8 17 8 24 0 100 5 10
    240613123506_dhid TCTAAGTAATTCTGCCAAGCAGTGGGTTAAGG(+AGGACA)GATCCTTTTACATTAAAATAAATAAATAACA NGG 10 8 17 8 24 0 100 5 10
    240613123504_1s1j GGTTATAACTGTCTGTAACTCCAGTGTCAGTG(+AGGACA)GATCCAATATTCTCTTCTGGTTCCACAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613123503_8yr6 GTAGGCACCTGTGGAACCAGAAGAGAATATTG(+AGGACA)GATCCACTGACACTGGAGTTACAGACAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613123501_sdj0 CTTCTGGGAGACTTGCTGGTACTGACTTCCTG(+AGGACA)GTACCTAGAGTTACCATGGAATCGGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613123500_4m6j ATAGAAACAAACCGATTCCATGGTAACTCTAG(+AGGACA)GTACCAGGAAGTCAGTACCAGCAAGTCTCCC NGG 10 8 17 8 24 0 100 5 10