Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613123527_uq47 TTTTAGTACCGACATATATTATTTTCATTGTG(+AGGACA)GATCCATTTTTTATTGCATGTTCAGATGTCA NGG 10 8 17 8 24 0 100 5 10
    240613123526_l5k8 GCAAATGACATCTGAACATGCAATAAAAAATG(+AGGACA)GATCCACAATGAAAATAATATATGTCGGTAC NGG 10 8 17 8 24 0 100 5 10
    240613123525_75lw GCTGTTTCAAGAGCATTAGGTACATGCACATG(+AGGACA)GTACCTTCCTAATTAATTGACCTGGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613123523_xx6p CAGCTCTTTTTCCAGGTCAATTAATTAGGAAG(+AGGACA)GTACCATGTGCATGTACCTAATGCTCTTGAA NGG 10 8 17 8 24 0 100 5 10
    240613123522_kbva ATAACTGTTAACTGCAACATATCTTTGGCAGG(+AGGACA)GATCCTCAGTCGTACATTTTGTCTTTAAATG NGG 10 8 17 8 24 0 100 5 10
    240613123520_1e9q AGAGGCATTTAAAGACAAAATGTACGACTGAG(+AGGACA)GATCCCTGCCAAAGATATGTTGCAGTTAACA NGG 10 8 17 8 24 0 100 5 10
    240613123519_rnfy TCTGAGAGCAGTGTAAACCTCACAGGGCTTTG(+AGGACA)GATCCCCTCGGACCCCCTTGGACACAGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613123517_l6un CCTTCTCCTCTGTGTCCAAGGGGGTCCGAGGG(+AGGACA)GATCCAAAGCCCTGTGAGGTTTACACTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613123516_kxi9 CAGTCCTACTGGAGATTCATTTTTGGCATCAG(+AGGACA)GTACCTGAGTTATTAACTTCATTCATTAAAT NGG 10 8 17 8 24 0 100 5 10
    240613123515_55dl CAGCTATTTAATGAATGAAGTTAATAACTCAG(+AGGACA)GTACCTGATGCCAAAAATGAATCTCCAGTAG NGG 10 8 17 8 24 0 100 5 10