Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115913_y4ib CAGCAGCCACTGGGAAGAAGAGGCACAACAAG(+AGGACA)GATCCATCCTCCCAGAGACCTTTCCAACTCC NGG 10 8 17 8 24 0 100 5 10
    240613115912_icoh TGGCAGGAGTTGGAAAGGTCTCTGGGAGGATG(+AGGACA)GATCCTTGTTGTGCCTCTTCTTCCCAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240613115911_qlx7 TCCTGGGCTTGGGCAGTGAGAGGAGCATCTAG(+AGGACA)GTACCTGTTGTGCCCCTAATAAAGTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613115909_wue7 TGTCATCCAACACTTTATTAGGGGCACAACAG(+AGGACA)GTACCTAGATGCTCCTCTCACTGCCCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613115908_72fu AATGAGTGCCTTCGCAGCCTGGTAGAAGAGAG(+AGGACA)GATCCGATTCGTCACCATCAGAATCCACTTT NGG 10 8 17 8 24 0 100 5 10
    240613115907_z78q TGGGGAAAGTGGATTCTGATGGTGACGAATCG(+AGGACA)GATCCTCTCTTCTACCAGGCTGCGAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613115906_auhe TGGTTCTAGCCCTTGACAGGTTGCTCCGCGAG(+AGGACA)GATCCCTGTAGCTCACACACTGGGCCTATGG NGG 10 8 17 8 24 0 100 5 10
    240613115904_36lv GCTGGCCATAGGCCCAGTGTGTGAGCTACAGG(+AGGACA)GATCCTCGCGGAGCAACCTGTCAAGGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240613115902_e6z3 AGGGGACAGTGGCACTAATGCCATAGGAAGGG(+AGGACA)GTACCTTCAGTACTTCCAGAGACAGGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240613115901_90ns GCCAGGCTGCCCTGTCTCTGGAAGTACTGAAG(+AGGACA)GTACCCCTTCCTATGGCATTAGTGCCACTGT NGG 10 8 17 8 24 0 100 5 10