Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115811_egp4 TGCAGTGGCCACAAAGGCCAGAGAGGCTGTTG(+AGGACA)GATCCCCTTGAGCTGGAGTTACAGGCGATCG NGG 10 8 17 8 24 0 100 5 10
    240613115809_8v5u GCAAACGATCGCCTGTAACTCCAGCTCAAGGG(+AGGACA)GATCCAACAGCCTCTCTGGCCTTTGTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613115807_uicx CATGGCTTGGGTTGTGGACCTTCTTCCCCATG(+AGGACA)GATCCAGAGTGGCACAGGGTCCTGCCCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613115806_bmb9 CTGTGCTCAGGGCAGGACCCTGTGCCACTCTG(+AGGACA)GATCCATGGGGAAGAAGGTCCACAACCCAAG NGG 10 8 17 8 24 0 100 5 10
    240613115805_x1d3 TGACCTGGCCAATGTACAGGCCTTTGTCTCAG(+AGGACA)GATCCTTGCCTGGTTTGCACAATAGGAAATG NGG 10 8 17 8 24 0 100 5 10
    240613115804_xt6v CACCACATTTCCTATTGTGCAAACCAGGCAAG(+AGGACA)GATCCTGAGACAAAGGCCTGTACATTGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613115801_fqll CAACACTGCACCACTGAACTGTGTCCCCCAGG(+AGGACA)GTACCATCACAGAGAATGGACAAAAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240613115800_hwrh GTGGGCTCATTTTTTGTCCATTCTCTGTGATG(+AGGACA)GTACCCTGGGGGACACAGTTCAGTGGTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613115759_fuwm TGCTAGTTGCTCTGAAAGATTGGGACCTCTTG(+AGGACA)GATCCCCCCCCCCATGGACTCTGATTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613115756_01ss ATAGACAGACAATCAGAGTCCATGGGGGGGGG(+AGGACA)GATCCAAGAGGTCCCAATCTTTCAGAGCAAC NGG 10 8 17 8 24 0 100 5 10