Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115326_g5vh CCAGATTGCAGTTAGATTTCTAAATTCCTGTG(+AGGACA)GTACCATGATTTGGCCTACTTCGTGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240613115325_17ax AAAGCCCTGGGTTGACTGACTCCCTCACTCAG(+AGGACA)GATCCTAAGAACAATGCTGACTGCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613115324_vypt AAGTAGAAAACTGCAGTCAGCATTGTTCTTAG(+AGGACA)GATCCTGAGTGAGGGAGTCAGTCAACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613115322_v9xc GTGCAAGCGCCATGGCACCCTGCTGTACCACG(+AGGACA)GTACCAAACTCAACTGTACAGTCAACAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613115321_k7aj CTCGCTGACTGTTGACTGTACAGTTGAGTTTG(+AGGACA)GTACCGTGGTACAGCAGGGTGCCATGGCGCT NGG 10 8 17 8 24 0 100 5 10
    240613115320_7vf8 CGTAGGCCGATGAGTGTAAGTAAAAGAGTGTG(+AGGACA)GTACCACTGGAAAAAACTCCAAACCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240613115318_aq99 TCGATGGCCCTGGTTTGGAGTTTTTTCCAGTG(+AGGACA)GTACCACACTCTTTTACTTACACTCATCGGC NGG 10 8 17 8 24 0 100 5 10
    240613115316_z6bd AGAAGGCCCCTCTGAGTATTTATTTGACCGGG(+AGGACA)GTACCAATGGTACAGCTATATATATGTACAG NGG 10 8 17 8 24 0 100 5 10
    240613115314_s6u6 TTACTCTGTACATATATATAGCTGTACCATTG(+AGGACA)GTACCCCGGTCAAATAAATACTCAGAGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613115312_jff1 AAGGACAGCTAGGCCAGGGGCCTTTGTCTGTG(+AGGACA)GTACCTAAGGAACCCTGAGTCAAACCAGTCC NGG 10 8 17 8 24 0 100 5 10