Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115421_gbgf TTTCTAAGCCACACAAGATGGGGTAAAAAGGG(+AGGACA)GTACCAACACCTCAATGGCAGTTAGACTTCC NGG 10 8 17 8 24 0 100 5 10
    240613115419_lo50 TGAGTGGAAGTCTAACTGCCATTGAGGTGTTG(+AGGACA)GTACCCCTTTTTACCCCATCTTGTGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613115417_cp63 ATGTGAATCCAGAGCTTGCTGGGGAGCCAGAG(+AGGACA)GTACCATTCAATGGCAGAGCTCTTCCCTAGC NGG 10 8 17 8 24 0 100 5 10
    240613115416_zj6c CTTATGCTAGGGAAGAGCTCTGCCATTGAATG(+AGGACA)GTACCTCTGGCTCCCCAGCAAGCTCTGGATT NGG 10 8 17 8 24 0 100 5 10
    240613115415_m4ha TCCGATGTCTGATGTGTCATCTTTTTATCTTG(+AGGACA)GATCCTTCCCTGGTTTGAAACTTTAAACTCT NGG 10 8 17 8 24 0 100 5 10
    240613115413_8pgp TTTCAAGAGTTTAAAGTTTCAAACCAGGGAAG(+AGGACA)GATCCAAGATAAAAAGATGACACATCAGACA NGG 10 8 17 8 24 0 100 5 10
    240613115412_n3jl CCTGTCCTTTAATTCTCAGAACCCCTTTCGAG(+AGGACA)GTACCTACATTGACTGGTAGTCGAGGGTCGA NGG 10 8 17 8 24 0 100 5 10
    240613115411_dt79 TGTAGTCGACCCTCGACTACCAGTCAATGTAG(+AGGACA)GTACCTCGAAAGGGGTTCTGAGAATTAAAGG NGG 10 8 17 8 24 0 100 5 10
    240613115410_qj47 TGCTTCTGCCAAATGACTAATATGGCCTGCTG(+AGGACA)GATCCCGAGCACACGGGGGCTTTGAGGTCAC NGG 10 8 17 8 24 0 100 5 10
    240613115408_69wc TGAGTGTGACCTCAAAGCCCCCGTGTGCTCGG(+AGGACA)GATCCAGCAGGCCATATTAGTCATTTGGCAG NGG 10 8 17 8 24 0 100 5 10