Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115451_hcpi GAGGGTTTTCAGTTGTGACAGCCCCACCCCCG(+AGGACA)GTACCATCTCTCTCCTCAATGTAGTCATGCA NGG 10 8 17 8 24 0 100 5 10
    240613115450_i3ab GTGCTTGCATGACTACATTGAGGAGAGAGATG(+AGGACA)GTACCGGGGGTGGGGCTGTCACAACTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240613115448_0op5 CTGGGGCCAATAAGCTGGCTTAGTAGGTAAAG(+AGGACA)GTACCTGCTGCCAAGCCTGATGCCCTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613115447_9npx GATTGAACTCAGGGCATCAGGCTTGGCAGCAG(+AGGACA)GTACCTTTACCTACTAAGCCAGCTTATTGGC NGG 10 8 17 8 24 0 100 5 10
    240613115446_8c80 TTGCCTGCGTTTATGTCTGTGTGAGAGTTTTG(+AGGACA)GATCCCCTAAAACGAGTTGCAAGCAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613115444_uut4 TAACTCACACCTGCTTGCAACTCGTTTTAGGG(+AGGACA)GATCCAAAACTCTCACACAGACATAAACGCA NGG 10 8 17 8 24 0 100 5 10
    240613115443_1ny8 AATAAGCCAGGCATGGTGCCTGGGGGCAGGAG(+AGGACA)GATCCTGACTAGAAAGCGATTGGTCTGCATA NGG 10 8 17 8 24 0 100 5 10
    240613115442_blg8 CTCACTATGCAGACCAATCGCTTTCTAGTCAG(+AGGACA)GATCCTCCTGCCCCCAGGCACCATGCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613115438_muy3 GTGTGTGTGTGTAAACCAGAGGGAAACCCCAG(+AGGACA)GTACCAGTCCTCTGATGCTGTCTACTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613115437_6at3 TGCAGGAAAGAGTAGACAGCATCAGAGGACTG(+AGGACA)GTACCTGGGGTTTCCCTCTGGTTTACACACA NGG 10 8 17 8 24 0 100 5 10