Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115436_1s2s ACCAGACATGTTCTAAGGCTGCAGTGCTGCAG(+AGGACA)GTACCCTCACCTTGGAGGTCATGCTGGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613115435_5zm7 TCTAGAACACCAGCATGACCTCCAAGGTGAGG(+AGGACA)GTACCTGCAGCACTGCAGCCTTAGAACATGT NGG 10 8 17 8 24 0 100 5 10
    240613115432_chzr TTCTTGGCATGAGCCTATCCTTATCGCCCTTG(+AGGACA)GATCCCAGTTTAACAAGACCTTTTTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613115430_riqg ATTGTCTTTAGAAAAAGGTCTTGTTAAACTGG(+AGGACA)GATCCAAGGGCGATAAGGATAGGCTCATGCC NGG 10 8 17 8 24 0 100 5 10
    240613115429_doom TGCATCTTCATCTTCATCACGACACCAGACAG(+AGGACA)GATCCAATCTTGGATGGAGACATTACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613115428_37r5 CCCTTCACTGGTAATGTCTCCATCCAAGATTG(+AGGACA)GATCCTGTCTGGTGTCGTGATGAAGATGAAG NGG 10 8 17 8 24 0 100 5 10
    240613115427_j4vs GTTGTTTGGTTTGTTTATTTCTGTGGTTCTGG(+AGGACA)GATCCAGCCCAGGGCCTTACATGTGCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240613115425_utpb TATTTGTCTAGCACATGTAAGGCCCTGGGCTG(+AGGACA)GATCCCAGAACCACAGAAATAAACAAACCAA NGG 10 8 17 8 24 0 100 5 10
    240613115423_9v7k GTTACAGCAGGGATTTGACAAAACACAGGGTG(+AGGACA)GATCCATGCAGTTTGGAAACTTAACTTTGAA NGG 10 8 17 8 24 0 100 5 10
    240613115422_ykxz GATCGTTCAAAGTTAAGTTTCCAAACTGCATG(+AGGACA)GATCCACCCTGTGTTTTGTCAAATCCCTGCT NGG 10 8 17 8 24 0 100 5 10