Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115522_276j GTGGAATTTCTCCAGGGTGGAATTTTAGACTG(+AGGACA)GTACCAGAAGTCGGCAAGGCGTGACTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613115520_n94q TGTGTCCAGCAGTCACGCCTTGCCGACTTCTG(+AGGACA)GTACCAGTCTAAAATTCCACCCTGGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613115519_6e3g CTACCACTAAGCTATACTTTCGAACTTTTTAG(+AGGACA)GATCCTTTGTGCTGGAGGAGAGCAAAGCAGG NGG 10 8 17 8 24 0 100 5 10
    240613115518_ssse AAGGCCCTGCTTTGCTCTCCTCCAGCACAAAG(+AGGACA)GATCCTAAAAAGTTCGAAAGTATAGCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613115516_6h2h ATCCAGGTTCTATGGCCAGCTGGCCACGAAGG(+AGGACA)GTACCTTGGTCCTAGGCAACCCACCTCTCGG NGG 10 8 17 8 24 0 100 5 10
    240613115515_dnt1 GAGAGCCGAGAGGTGGGTTGCCTAGGACCAAG(+AGGACA)GTACCCTTCGTGGCCAGCTGGCCATAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613115513_r9fb CATATCAAGGACAGGGTGAGGAAGTGGCCGTG(+AGGACA)GATCCAGCTGAGAGTGGCAGCCTCGGGACTG NGG 10 8 17 8 24 0 100 5 10
    240613115512_d257 GCATCCAGTCCCGAGGCTGCCACTCTCAGCTG(+AGGACA)GATCCACGGCCACTTCCTCACCCTGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613115510_vpb6 GGCTCATCACGCCAACCATGCCCAGCTCTGGG(+AGGACA)GTACCCAGCCTTGAATGGGAGAGAAAAGGCC NGG 10 8 17 8 24 0 100 5 10
    240613115508_n795 CCTGAGGCCTTTTCTCTCCCATTCAAGGCTGG(+AGGACA)GTACCCCAGAGCTGGGCATGGTTGGCGTGAT NGG 10 8 17 8 24 0 100 5 10