Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115609_zns1 ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+AGGACA)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613115608_5t18 GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+AGGACA)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613115607_02dz TCTACATTTTAATTTTTATGATTCCTGATGTG(+AGGACA)GATCCATTACAAACTTCTCAGAAGTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613115605_crie ATGCACTCCAAACTTCTGAGAAGTTTGTAATG(+AGGACA)GATCCACATCAGGAATCATAAAAATTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613115602_35o8 GGAAGAAAGGTGGTGTCCTTCCGGACTCCGGG(+AGGACA)GATCCAGGCCTGAATATGGGAGGCACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240613115601_jktb CTGAGCCTGGGTGCCTCCCATATTCAGGCCTG(+AGGACA)GATCCCCGGAGTCCGGAAGGACACCACCTTT NGG 10 8 17 8 24 0 100 5 10
    240613115559_fdze AAAAATTACTAAAACCATGCAAAATGATTGAG(+AGGACA)GATCCCAAAGGCTTTCTTAATGTACACTAGT NGG 10 8 17 8 24 0 100 5 10
    240613115558_r5c4 TAGATACTAGTGTACATTAAGAAAGCCTTTGG(+AGGACA)GATCCTCAATCATTTTGCATGGTTTTAGTAA NGG 10 8 17 8 24 0 100 5 10
    240613115557_hide TCTTGGATGGACTTCTAAAATATATGTTGATG(+AGGACA)GATCCCTTTTTTATTTTGTGTTTTTTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613115555_mfbs ACTATATTAAAAAAAACACAAAATAAAAAAGG(+AGGACA)GATCCATCAACATATATTTTAGAAGTCCATC NGG 10 8 17 8 24 0 100 5 10