Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115640_4n8q TGTGTATGTCTGTGTACCAGAAGACGACATTG(+AGGACA)GATCCCATAGATCTGTTGTATGGATGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613115638_0zow GACTTACAGCCATCCATACAACAGATCTATGG(+AGGACA)GATCCAATGTCGTCTTCTGGTACACAGACAT NGG 10 8 17 8 24 0 100 5 10
    240613115636_vfed GTAAAGTAGTAGCTAACCCCTCCAAGGCCCAG(+AGGACA)GATCCTAGTTCCAGTGTTACAGAAATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240613115635_qi7u AAAAGGAAACATTTCTGTAACACTGGAACTAG(+AGGACA)GATCCTGGGCCTTGGAGGGGTTAGCTACTAC NGG 10 8 17 8 24 0 100 5 10
    240613115633_uri4 CACTTTACTGTTTCAATGGTAATAATGCACCG(+AGGACA)GTACCTCAATTCAACTGCTACAAATAAATGT NGG 10 8 17 8 24 0 100 5 10
    240613115632_ggde TTTTGACATTTATTTGTAGCAGTTGAATTGAG(+AGGACA)GTACCGGTGCATTATTACCATTGAAACAGTA NGG 10 8 17 8 24 0 100 5 10
    240613115631_57j8 ATGTATCCTTACTTTGACAATTAGACACACAG(+AGGACA)GTACCAGGAAAAAAGGAATTGACTTTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613115629_99em TTCCAGCCTGAAAGTCAATTCCTTTTTTCCTG(+AGGACA)GTACCTGTGTGTCTAATTGTCAAAGTAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613115628_1pc3 TGGTTTGCATTGACCGCTTTCCTCCTTGACTG(+AGGACA)GTACCTTTCCCGTCTTTACGTATGGGAATTG NGG 10 8 17 8 24 0 100 5 10
    240613115627_mdmo GAGGGCAATTCCCATACGTAAAGACGGGAAAG(+AGGACA)GTACCAGTCAAGGAGGAAAGCGGTCAATGCA NGG 10 8 17 8 24 0 100 5 10