Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115654_pk3m TGGCTTACAACTGTCTATACCTCAGTTCCAGG(+AGGACA)GATCCAGTGCCACCTTATGGTTCCCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613115653_sdmv TGCATGCCTGTGGGAACCATAAGGTGGCACTG(+AGGACA)GATCCCTGGAACTGAGGTATAGACAGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240613115652_ztvf CTATTTAACTTTCCCTTAAATTTTAAGATTTG(+AGGACA)GATCCCATGTATGTCATCATGTCCCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613115650_zlp9 AAATATTCTAGGGGACATGATGACATACATGG(+AGGACA)GATCCAAATCTTAAAATTTAAGGGAAAGTTA NGG 10 8 17 8 24 0 100 5 10
    240613115649_a3up GTAAAGAGCATGTAAATACCACAGGAAGACAG(+AGGACA)GATCCCTGGAGACATTGAAGGAACAAGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613115647_8r1o CCTCCCTCTCTTGTTCCTTCAATGTCTCCAGG(+AGGACA)GATCCTGTCTTCCTGTGGTATTTACATGCTC NGG 10 8 17 8 24 0 100 5 10
    240613115646_3kpi TCGTAGTCGCTAAAATTCACTCAATTCACATG(+AGGACA)GATCCCAGCTGTGTGTTGTAAGGCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613115644_xetv TCCACTGACTCAGCCTTACAACACACAGCTGG(+AGGACA)GATCCATGTGAATTGAGTGAATTTTAGCGAC NGG 10 8 17 8 24 0 100 5 10
    240613115643_mhy1 TCCACTTTGAACGTTTTCAGAAATCCCATAAG(+AGGACA)GATCCTGGAGTGATAGATGATTTCTGTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613115641_or37 TTCACCAAGACAGAAATCATCTATCACTCCAG(+AGGACA)GATCCTTATGGGATTTCTGAAAACGTTCAAA NGG 10 8 17 8 24 0 100 5 10