Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115625_fzls GAGCGCATCCCAGCATGGAGCAGGTAGAGTGG(+AGGACA)GTACCAAGGCACACCCCTAGCTCAGAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613115623_4157 GCCAATAGCCTCTGAGCTAGGGGTGTGCCTTG(+AGGACA)GTACCCACTCTACCTGCTCCATGCTGGGATG NGG 10 8 17 8 24 0 100 5 10
    240613115622_85ry CGAGAGGTTTTCACAAACCGAGTCAAAACAAG(+AGGACA)GTACCAGTTTTGAGCTCACATCAACTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613115621_ty8x ACACTTAAAAAGTTGATGTGAGCTCAAAACTG(+AGGACA)GTACCTTGTTTTGACTCGGTTTGTGAAAACC NGG 10 8 17 8 24 0 100 5 10
    240613115620_nr95 TCTCTGTCTTGAGAGACCAGAAGAGAGCATTG(+AGGACA)GATCCCATTACAGATGATTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613115618_wyy2 ACCACATGGTGGCTCACAATCATCTGTAATGG(+AGGACA)GATCCAATGCTCTCTTCTGGTCTCTCAAGAC NGG 10 8 17 8 24 0 100 5 10
    240613115616_6tue ATTGCAAGCAAGGGATTAAAGATTTGTCTTGG(+AGGACA)GTACCAAATCACATAACGAGGATTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613115615_nm64 GTTAATCCTTTGAATCCTCGTTATGTGATTTG(+AGGACA)GTACCCAAGACAAATCTTTAATCCCTTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613115614_szyt TGGATTCGTATGGCTAACGTCAGTGAATCAAG(+AGGACA)GATCCCAGGGGATGCCCATTGCTGGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240613115611_i1gp AACTGAGATGCCCAGCAATGGGCATCCCCTGG(+AGGACA)GATCCTTGATTCACTGACGTTAGCCATACGA NGG 10 8 17 8 24 0 100 5 10