Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115537_6gb4 AGGTGGTGTCTGCATTCCAACCACTGAATCCG(+AGGACA)GATCCCATTTGTGAATAAGCCATTCAGACTC NGG 10 8 17 8 24 0 100 5 10
    240613115535_jd3q AATATGAGTCTGAATGGCTTATTCACAAATGG(+AGGACA)GATCCGGATTCAGTGGTTGGAATGCAGACAC NGG 10 8 17 8 24 0 100 5 10
    240613115534_ch8t GGAAACTGAAGTGTGTGCACACTTGAAGGAAG(+AGGACA)GATCCCGAGTTTTCATATATTTTTAATATAT NGG 10 8 17 8 24 0 100 5 10
    240613115532_0p7q TCTTAATATATTAAAAATATATGAAAACTCGG(+AGGACA)GATCCTTCCTTCAAGTGTGCACACACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240613115531_k9pw ACAGACCAGGTAAAGAGAAAGCCCTAGTACAG(+AGGACA)GTACCTTGCAATCAACCATGCTTGGTTCAAC NGG 10 8 17 8 24 0 100 5 10
    240613115530_wsf7 TCAAGGTTGAACCAAGCATGGTTGATTGCAAG(+AGGACA)GTACCTGTACTAGGGCTTTCTCTTTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240613115528_6svr CAGAGCCAACAGCTTCTCATTCTAAAGCAAGG(+AGGACA)GATCCCTAGAGTTTGGAGCTTCATGTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613115527_6cqs CCTAACCCCAACATGAAGCTCCAAACTCTAGG(+AGGACA)GATCCCTTGCTTTAGAATGAGAAGCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613115525_te7w GCCTCTGTGTAGTCTGGATCTACTGACACTGG(+AGGACA)GTACCAGGGTTTCCTTCTGTTGACTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240613115523_pypm GCTGCAATCACAGTCAACAGAAGGAAACCCTG(+AGGACA)GTACCCAGTGTCAGTAGATCCAGACTACACA NGG 10 8 17 8 24 0 100 5 10