Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115340_i3rl CCTCCCTGAACCCACTCCCGTTCTGGTAGCTG(+AGGACA)GATCCCCTCCCTCTGATTTTGATATCATAGT NGG 10 8 17 8 24 0 100 5 10
    240613115339_to7h TTGGACAGCCAGGGCTACACAGAGAGATCCTG(+AGGACA)GATCCCGTCTTCCAAAAAAAAAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240613115337_5wbg CTTTCTTTCTTTTTTTTTTTTTTGGAAGACGG(+AGGACA)GATCCAGGATCTCTCTGTGTAGCCCTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613115336_rn0s TCAGAAACTTCAGGCTCTGCTTGGAAAACTTG(+AGGACA)GTACCACAAATGGGTGTCAAAGGTCATGTGT NGG 10 8 17 8 24 0 100 5 10
    240613115335_bbcu TTGTTACACATGACCTTTGACACCCATTTGTG(+AGGACA)GTACCAAGTTTTCCAAGCAGAGCCTGAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613115334_cbx0 CTGGCCCCAGGCTGGGGTCCTATGCAAAGCAG(+AGGACA)GATCCCACTAATGGGATTCAGGCAGAAGTGT NGG 10 8 17 8 24 0 100 5 10
    240613115332_4fmu GGTAGACACTTCTGCCTGAATCCCATTAGTGG(+AGGACA)GATCCTGCTTTGCATAGGACCCCAGCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613115330_5cbl CGACTCAAAGGGCCTTCTTGCTGGGGCCTCTG(+AGGACA)GATCCTCTGTCCTGGGGCCGGAAGGCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613115329_xys5 TCCGGCCCCTGCCTTCCGGCCCCAGGACAGAG(+AGGACA)GATCCAGAGGCCCCAGCAAGAAGGCCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613115328_5ddl TTGTCATTCATCACGAAGTAGGCCAAATCATG(+AGGACA)GTACCACAGGAATTTAGAAATCTAACTGCAA NGG 10 8 17 8 24 0 100 5 10