Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613185211_uf1l ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185209_lis6 TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240613185207_2epw ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185206_xcev GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240613185204_75c7 ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185202_itqv GATTGAAAAAAATGTTTATTTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240613185200_qzvv ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAAATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185159_7nrp TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAAAACCTCAGTA NGG 10 8 17 8 24 0 100 5 10
    240613185157_728q AAAGATACTGAGGTTTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185155_8lxs TTGAAAAAAAAATGTTTGTTTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAAAACCTCAGTA NGG 10 8 17 8 24 0 100 5 10