Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".
  • Substitution is indicated by backslash "/", and the example is used to replace A by T.
  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position
  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.
  • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613185120_yfp9 TGATGGGTCTCTCCAAGGACAGGAGAGAGCAG(+AGGACA)GTACCACATCCTCTGCTTGAGATATTGTATG NGG 10 8 17 8 24 0 100 5 10
    240613185118_2qri GATTGAAAAAAATGTTTATCTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAACCTCATTATC NGG 10 8 17 8 24 0 100 5 10
    240613185116_w0a6 ACAAAGATAATGAGGTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAGATAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185115_qtbg ATTGAAAAAAAATGTTTGTTTTCCTGTGTTGG(+AGGACA)GTACCTATGGGTCTTTGACAACCTCAGTATC NGG 10 8 17 8 24 0 100 5 10
    240613185113_hxlk ACAAAGATACTGAGGTTGTCAAAGACCCATAG(+AGGACA)GTACCCAACACAGGAAAACAAACATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613185112_yz0g TGATCTTGTAATAGTTCTATCTTTAGATCTTG(+AGGACA)GATCCAACTTGCTGCATCTTTCTGTCTGTAG NGG 10 8 17 8 24 0 100 5 10
    240613185110_r2wu AGCTACTACAGACAGAAAGATGCAGCAAGTTG(+AGGACA)GATCCAAGATCTAAAGATAGAACTATTACAA NGG 10 8 17 8 24 0 100 5 10
    240613185108_h4x5 CTGCAGAGACTTGATGTGCCTGGTGGGAGGGG(+AGGACA)GTACCACCCTCTCAGAGAAAAAGGGGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613185106_s96h ATAACTCCCTCCCCTTTTTCTCTGAGAGGGTG(+AGGACA)GTACCCCCTCCCACCAGGCACATCAAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613185104_mptv GTGATCAAGGCAGAGAAGGCTCATTGTGGGTG(+AGGACA)GTACCATCCCTGGGCTGGTAGTTTTGGGATC NGG 10 8 17 8 24 0 100 5 10