Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613185045_uvcl TTAGACAACCTATACCTTTAGTTATTGCCTAG(+AGGACA)GATCCTGCCCATAATTTCCCCTGTTCTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613185043_o6o1 CTGAAGAATGTCACCACCTAGGACATGCCTTG(+AGGACA)GTACCTGAAGTTTTCATAAAGGTTTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240613185042_80so TTCATTTATGGAAACCTTTATGAAAACTTCAG(+AGGACA)GTACCAAGGCATGTCCTAGGTGGTGACATTC NGG 10 8 17 8 24 0 100 5 10
    240613185040_z3p0 GGTGTCAGCATTTGGAAGCTGATTATAGGATG(+AGGACA)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613185038_iroy AGATGGACCATCTAGTGATTGCCATATGCAGG(+AGGACA)GATCCATCCTATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613185037_a1ub TACTGTGATTTTCATAACCCGTTGCCTTTTTG(+AGGACA)GTACCAGAGCTACGTGGTTTGAATCCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240613185035_4l3b CATGTAGCCAGGATTCAAACCACGTAGCTCTG(+AGGACA)GTACCAAAAAGGCAACGGGTTATGAAAATCA NGG 10 8 17 8 24 0 100 5 10
    240613185033_uk8r GGAGTCAAGGACTATAGTCAGGAGACCCCCAG(+AGGACA)GTACCTGGTTCAGAGCAGTCCGGGATGATAG NGG 10 8 17 8 24 0 100 5 10
    240613185031_ce1n TCGTCCTATCATCCCGGACTGCTCTGAACCAG(+AGGACA)GTACCTGGGGGTCTCCTGACTATAGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613185029_lcqz GTGTAATTGACCTGAAACATATACACGAAAAG(+AGGACA)GTACCCATTTCTTCTAATGCCTTTAAAAATA NGG 10 8 17 8 24 0 100 5 10