Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184950_boyd TTGGATTGAATAGGTGAGTCCAGGCTTCAGAG(+AGGACA)GATCCAAGGGAAAGTTGTTCTTGACCTTGCC NGG 10 8 17 8 24 0 100 5 10
    240613184948_6uwv GACAGTAAAGCACTGTCTGCAACCCAGAAATG(+AGGACA)GTACCATTTATACGACAGGCTTGATGTGGAC NGG 10 8 17 8 24 0 100 5 10
    240613184947_lm1a TCAGAGTCCACATCAAGCCTGTCGTATAAATG(+AGGACA)GTACCATTTCTGGGTTGCAGACAGTGCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613184946_jxvc CTAGAAGCACCCCCCACCCCCAGCCTTCAAAG(+AGGACA)GTACCACAATCAGGGTATGCTATTTTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613184945_raaf TAATAAGCAGAAAATAGCATACCCTGATTGTG(+AGGACA)GTACCTTTGAAGGCTGGGGGTGGGGGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613184943_jt74 GAACCTTACAAGTAGAGATTGATAAAGAGAAG(+AGGACA)GTACCCTACCTCCTCTCTCTCTCTCGCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613184942_c69r AGAGAGAGAGCGAGAGAGAGAGAGGAGGTAGG(+AGGACA)GTACCTTCTCTTTATCAATCTCTACTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613184938_q7f6 CCCTCAACTGCCTATTAATTAAAACTCCAGGG(+AGGACA)GATCCAATGTTCTCTTCTGACTTCTGCATTC NGG 10 8 17 8 24 0 100 5 10
    240613184937_slh7 CATGTGAATGCAGAAGTCAGAAGAGAACATTG(+AGGACA)GATCCCCTGGAGTTTTAATTAATAGGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240613184936_j495 GAAAAGGAGGTTCAGCGACAGACCCAAAGTAG(+AGGACA)GATCCAGATCAAGGAGATGTCCCAAGGCCTC NGG 10 8 17 8 24 0 100 5 10