Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184915_9nk7 ATTTTCAACTAGTAAATGGTTTGTAATTTTTG(+AGGACA)GATCCCCCCACCCCCGAATATGGTCAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613184913_v6la TTGTTGTTTTTTTTTTTTCTTACTAAAAGTGG(+AGGACA)GATCCTTTTAGGCAGGATTTTATTCTGTAGC NGG 10 8 17 8 24 0 100 5 10
    240613184912_3emf CTTTGGCTACAGAATAAAATCCTGCCTAAAAG(+AGGACA)GATCCCACTTTTAGTAAGAAAAAAAAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613184910_ossf TAACCTCAGCCCTGAGTTCAGGGAGACAGGTG(+AGGACA)GATCCCATGGACTCACTGGCTGGATAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613184908_xrmq TTGATTAGCTTATCCAGCCAGTGAGTCCATGG(+AGGACA)GATCCACCTGTCTCCCTGAACTCAGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613184906_06ew TGGTATGAGGCAAAGCCTCTTGAACACAAATG(+AGGACA)GTACCCTCTTCATAATGGCTTTATTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613184905_wupj GGATCCAAAGGAATAAAGCCATTATGAAGAGG(+AGGACA)GTACCATTTGTGTTCAAGAGGCTTTGCCTCA NGG 10 8 17 8 24 0 100 5 10
    240613184903_lgqv GGTACCCTCTTCATAATGGCTTTATTCCTTTG(+AGGACA)GATCCTATTAAAGATTCCCTGCAGCTTGTAC NGG 10 8 17 8 24 0 100 5 10
    240613184901_jokg CACTGGTACAAGCTGCAGGGAATCTTTAATAG(+AGGACA)GATCCAAAGGAATAAAGCCATTATGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613184900_q3ep GCCTCACAACACTCCTGTGAGGTAGATATGTG(+AGGACA)GATCCCCATTTCGAGATGGGTTAATAGAGGC NGG 10 8 17 8 24 0 100 5 10