Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184810_4pek ATTTCTTGCTGAGACCAACAAAGTAGCATATG(+AGGACA)GATCCAGGGTGCAACATGACCCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613184809_2g1z CTTTTTTTTTCTTCTTTTTGAAACAATGCTTG(+AGGACA)GATCCTGTTCTTGGGCACATGGGGCCATTTG NGG 10 8 17 8 24 0 100 5 10
    240613184808_a0bb GGGTCCAAATGGCCCCATGTGCCCAAGAACAG(+AGGACA)GATCCAAGCATTGTTTCAAAAAGAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613184806_pwih TCTAATATAGGAAAATAGGAAATCCACCAGTG(+AGGACA)GTACCATCTCTCAACATAAACATATGTACTA NGG 10 8 17 8 24 0 100 5 10
    240613184804_84hc TCATCTAGTACATATGTTTATGTTGAGAGATG(+AGGACA)GTACCACTGGTGGATTTCCTATTTTCCTATA NGG 10 8 17 8 24 0 100 5 10
    240613184803_rbej CCAAAACCACATGGGATTTTGACAACAAACTG(+AGGACA)GATCCTTGCTACAATATTAATGTTCTATTCT NGG 10 8 17 8 24 0 100 5 10
    240613184802_wfz6 AAGAAAGAATAGAACATTAATATTGTAGCAAG(+AGGACA)GATCCAGTTTGTTGTCAAAATCCCATGTGGT NGG 10 8 17 8 24 0 100 5 10
    240613184801_7zbc CCCCACTCCATTTGACTGTCTCTTCTTCTGTG(+AGGACA)GATCCTGCAGCAGAGTTTTATTTCAGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613184759_2cf3 ATGAGTAAGACTGAAATAAAACTCTGCTGCAG(+AGGACA)GATCCACAGAAGAAGAGACAGTCAAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240613184758_okxy TTTCTGGCTCCTAAACGCCTTTGAGCTTGTTG(+AGGACA)GTACCCAGAGAAATAAACTAAAATTGTATGA NGG 10 8 17 8 24 0 100 5 10