Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184735_wlrl AAATCTCTTTCCCAGGTTCAGTGCTACACAAG(+AGGACA)GATCCTAATTTTGAATACGTAGGATTCCACC NGG 10 8 17 8 24 0 100 5 10
    240613184734_1z63 CTGTTTCACAAGCATCTGCTCTTGGCCTTTCG(+AGGACA)GTACCACCTCCTGATTCTCAACCACCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613184732_43l0 CCAGACCCCAGGTGGTTGAGAATCAGGAGGTG(+AGGACA)GTACCGAAAGGCCAAGAGCAGATGCTTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613184731_i5lf AAATAATTATGGCCACAAAATTATATGTGATG(+AGGACA)GATCCACTGCTTTTTTATATTCTTTTCAATA NGG 10 8 17 8 24 0 100 5 10
    240613184730_f530 AAACATATTGAAAAGAATATAAAAAAGCAGTG(+AGGACA)GATCCATCACATATAATTTTGTGGCCATAAT NGG 10 8 17 8 24 0 100 5 10
    240613184728_7xbl TATGAAACATTGAAACAACAGGCCCAGGAGGG(+AGGACA)GATCCAGCCTCTAATCCAGGTTTGATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240613184726_37kp ACAAATGCAAATCAAACCTGGATTAGAGGCTG(+AGGACA)GATCCCCTCCTGGGCCTGTTGTTTCAATGTT NGG 10 8 17 8 24 0 100 5 10
    240613184725_hvit CTCAGTATGTATTTGACTGTATGAAATAAATG(+AGGACA)GATCCTTCTATGTGTCTCTATATATGTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613184723_1k9q AGAGAATAGACATATATAGAGACACATAGAAG(+AGGACA)GATCCATTTATTTCATACAGTCAAATACATA NGG 10 8 17 8 24 0 100 5 10
    240613184722_knb3 CTGCAAAGGGAAGGAGTACAGGAAGCGCCATG(+AGGACA)GATCCTACAGTGTTAGTTTGTCAGCTAGCCA NGG 10 8 17 8 24 0 100 5 10